Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179)

Name: Anti-Lipoamide Dehydrogenase Antibody [ARC1224]
Brand: Antibodies.com
SKU: A309179
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1224] antibody to Lipoamide Dehydrogenase for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Lipoamide Dehydrogenase Antibody [ARC1224]

Description

Rabbit monoclonal [ARC1224] antibody to Lipoamide Dehydrogenase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-500 of human DLDH/DLD (P09622).

Sequence

WVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1224

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dehydrogenase complex, E3 component, Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, dld, DLDD, DLDH, DLDH_HUMAN, E3, E3 branched chain aplha-keto acid, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, GCSL, Glycine cleavage system L protein, Glycine cleavage system protein L, LAD, Lipoamide reductase, Lipoyl dehydrogenase, mitochondrial, OTTHUMP00000206744, OTTHUMP00000206746, OTTHUMP00000206748, OTTHUMP00000206749, PHE 3, PHE3, Pyruvate dehydrogenase component E3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179)

Western blot analysis of extracts of various cell lines, using Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Lipoamide Dehydrogenase Antibody [ARC1224] (A309179) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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