This antibody recognises members of the lymphocyte activation protein-6 (Ly-6) multi-gene family. Specifically, it recognises Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of sodium azide is recommended prior to use in functional assays.
Applications
Flow Cytometry, WB, IHC-Fr, IP
Reactivity
Mouse
Immunogen
IL-2 dependent mouse T cell line CTL-L.
Host
Rat
Clonality
Monoclonal
Clone ID
D7
Isotype
IgG2a
Conjugate
Unconjugated
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Borate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse Ly-6A/E antibody, clone D7 recognizes members of the lymphocyte activation protein-6 (Ly-6) multi-gene family. Specifically, it recognizes Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of sodium azide is recommended prior to use in functional assays.
