Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)

Name: Anti-M6PR (cation independent) Antibody [ARC51203]
Brand: Antibodies.com
SKU: A308940

Enlarge Image

This product is discontinued

Anti-M6PR (cation independent) Antibody [ARC51203] (A308940) has been discontinued and is no longer available.

View all Anti-M6PR (cation independent) Antibodies.

Specifications

Name

Anti-M6PR (cation independent) Antibody [ARC51203]

Description

Rabbit monoclonal [ARC51203] antibody to M6PR (cation independent).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2140-2300 of human Cation-independent M6PR (IGF2R) (NP_000867.3).

Sequence

TNPINGKSFSLGDIYFKLFRASGDMRTNGDNYLYEIQLSSITSSRNPACSGANICQVKPNDQHFSRKVGTSDKTKYYLQDGDLDVVFASSSKCGKDKTKSVSSTIFFHCDPLVEDGIPEFSHETADCQYLFSWYTSAVCPLGVGFDSENPGDDGQMHKGLS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51203

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

274 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

300 kDa mannose 6 phosphate receptor, 300 kDa mannose 6-phosphate receptor, Cation independent mannose 6 phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CD222 antigen, CI Man 6 P receptor, CI Man-6-P receptor, CI MPR, CI-M6PR, CI-MPR, CIMPR, IGF 2 receptor, IGF 2R, IGF II receptor, IGF-II receptor, IGF2 receptor, Igf2r, Insulin like growth factor 2 receptor, Insulin like growth factor II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, mannose 6 phosphate receptor, mannose 6 phosphate receptor, cation independent, MPR 300, MPR300, MPRI, MPRI_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)

Western blot analysis of extracts of various cell lines, using Anti-M6PR (cation independent) Antibody [ARC51203] (A308940) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 2: Western Blot - Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)

Western blot analysis of extracts of Rat liver, using Anti-M6PR (cation independent) Antibody [ARC51203] (A308940) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 3: Western Blot - Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-M6PR (cation independent) Antibody [ARC51203] (A308940) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-M6PR (cation independent) Antibody [ARC51203] (A308940) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Show more images

Publishing research using Anti-M6PR (cation independent) Antibody [ARC51203] (A308940)? Please let us know so that we can list the citation on this page.