Anti-Macrophages/Monocytes/Granulocytes Antibody [MAC387] (APC) (A281023)

$515

Mouse monoclonal [MAC387] antibody to Macrophages/Monocytes/Granulocytes (APC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Macrophages/Monocytes/Granulocytes Antibody [MAC387] (APC)
Description
Mouse monoclonal [MAC387] antibody to Macrophages/Monocytes/Granulocytes (APC).
Specificity
This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.
Applications
Flow Cytometry
Dilutions
FC 1: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Horse, Pig, Dog, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Cat, Cynomolgus monkey, Rhesus Monkey, Goat, Fallow deer, Pygmy hippopotamus, Mink, Marmoset
Immunogen
Human monocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MAC387
Isotype
IgG1
Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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