This antibody recognises human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a ~60 kDa transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, recognises the mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1 (Leung, E. et al. 2004).
Applications
WB, IHC-P, Flow Cytometry, ELISA
Dilutions
Flow Cytometry: 1:10 - 1:100, IHC-P: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Camel
Immunogen
Recombinant soluble MAdCAM-1-fc fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
17F5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 60 kDa.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a ~60 kDa transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes the mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1 (Leung, E. et al. 2004).
