Western blot analysis of extracts of various cell lines, using Anti-MAdCAM1 Antibody (A9125) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MAdCAM1 Antibody (A9125) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative products to Anti-MAdCAM1 Antibody (A9125)
Recombinant human monoclonal antibody to MAdCAM1 for use as a research grade Ontamalimab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to MAdCAM1 for use as a research grade Ontamalimab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
