Anti-MAdCAM1 Antibody (A9125)

Name: Anti-MAdCAM1 Antibody
Brand: Antibodies.com
SKU: A9125

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This product is discontinued

Anti-MAdCAM1 Antibody (A9125) has been discontinued and is no longer available.

View all Anti-MAdCAM1 Antibodies.

Specifications

Name

Anti-MAdCAM1 Antibody

Description

Rabbit polyclonal antibody to MAdCAM1.

Applications

WB, IHC

Dilutions

WB: 1:200-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MADCAM1 (NP_570116.2).

Sequence

GRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Addressin mucosal, hMAdCAM 1, hMAdCAM-1, MACAM1, MAdCAM 1, MAdCAM-1, MADCA_HUMAN, Mucosal addressin cell adhesion molecule 1, Mucosal vascular addressin cell adhesion molecule 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-MAdCAM1 Antibody (A9125)

Western blot analysis of extracts of various cell lines, using Anti-MAdCAM1 Antibody (A9125) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-MAdCAM1 Antibody (A9125)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MAdCAM1 Antibody (A9125) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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