Anti-MAP1LC3A Antibody [ARC0319] (A306460)

Name: Anti-MAP1LC3A Antibody [ARC0319]
Brand: Antibodies.com
SKU: A306460

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This product is discontinued

Anti-MAP1LC3A Antibody [ARC0319] (A306460) has been discontinued and is no longer available.

View all Anti-MAP1LC3A Antibodies.

Specifications

Name

Anti-MAP1LC3A Antibody [ARC0319]

Description

Rabbit monoclonal [ARC0319] antibody to MAP1LC3A.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 42-121 of human LC3A (Q9H492).

Sequence

KQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0319

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, MAP1 light chain 3 like protein 1, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, Microtubule associated proteins 1A/1B light chain 3, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A and 1B, light chain 3, Microtubule-associated proteins 1A/1B light chain 3A, MLP3A_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MAP1LC3A Antibody [ARC0319] (A306460)

Western blot analysis of extracts of various cell lines, using Anti-MAP1LC3A Antibody [ARC0319] (A306460) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-MAP1LC3A Antibody [ARC0319] (A306460)

Western blot analysis of extracts of HeLa cells, using Anti-MAP1LC3A Antibody [ARC0319] (A306460) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-MAP1LC3A Antibody [ARC0319] (A306460)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-MAP1LC3A Antibody [ARC0319] (A306460) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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