Anti-MDC1 Antibody (A295223)

Name: Anti-MDC1 Antibody
Brand: Antibodies.com
SKU: A295223
Price: 475.0 USD
Availability: InStock

This product is identical to Abcam (ab11171). Discover more for less.

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$475

Rabbit polyclonal antibody to MDC1 for WB, IP and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MDC1 Antibody

Description

Rabbit polyclonal antibody to MDC1.

Applications

WB, IP, IHC

Dilutions

IHC: 1:500-1:2,000, IP: 2-5 µg / 1 mg lysate, WB: 1:5,000-1:25,000

Reactivity

Human

Immunogen

Synthetic peptide within amino acids 1375-1425 of human MDC1 (NP_055456. 1).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

Homologue to Drosophila photoreceptor protein calphotin, MDC 1, MDC1_HUMAN, Mediation of DNA damage checkpoint 1, Mediator of DNA damage checkpoint 1, Mediator of DNA damage checkpoint protein 1, NFBD 1, NFBD1, Nuclear factor with BRCT domains 1, Nuclear Factor with BRCT Domains Protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunohistochemistry - Anti-MDC1 Antibody (A295223)

Sample: FFPE section of human breast carcinoma. Antibody: Anti-MDC1 Antibody (A295223) was used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

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Figure 2: Immunoprecipitation - Anti-MDC1 Antibody (A295223)

Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Anti-MDC1 Antibody (A295223) was used at 1 µg/ml for WB and used for IP at 3 µg/mg lysate. MDC1 was also immunoprecipitated by another lot of Anti-MDC1 Antibody (A295223). Detection: Chemiluminescence with exposure time of 3 seconds.

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Figure 3: Western Blot - Anti-MDC1 Antibody (A295223)

Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Anti-MDC1 Antibody (A295223) was used at 0.1 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.

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