Anti-Meckelin Antibody [ARC3051] (A309404)

Name: Anti-Meckelin Antibody [ARC3051]
Brand: Antibodies.com
SKU: A309404

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This product is discontinued

Anti-Meckelin Antibody [ARC3051] (A309404) has been discontinued and is no longer available.

View all Anti-Meckelin Antibodies.

Specifications

Name

Anti-Meckelin Antibody [ARC3051]

Description

Rabbit monoclonal [ARC3051] antibody to Meckelin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of Human Meckelin.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC3051

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

112 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

JBTS6, Meckel syndrome type 3 protein, MKS3, NPHP11, TMEM67, TNEM67, Transmembrane protein 67

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Meckelin Antibody [ARC3051] (A309404)

Western blot analysis of extracts of various cell lines, using Anti-Meckelin Antibody [ARC3051] (A309404) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Meckelin Antibody [ARC3051] (A309404)

Western blot analysis of extracts of PC-12 cells, using Anti-Meckelin Antibody [ARC3051] (A309404) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Meckelin Antibody [ARC3051] (A309404)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Meckelin Antibody [ARC3051] (A309404) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Meckelin Antibody [ARC3051] (A309404)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Meckelin Antibody [ARC3051] (A309404) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.