Anti-MED21 Antibody [PCRP-MED21-4B5] (A277849)

Anti-MED21 Antibody [PCRP-MED21-4B5]

Mouse monoclonal [PCRP-MED21-4B5] antibody to MED21.

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

ELISA, IP, Flow Cytometry, IF, WB

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml

Human

Recombinant full-length human MED21 protein.

Mouse

Monoclonal

PCRP-MED21-4B5

IgG1

Protein A/G chromatography.

200 µg/ml

15.5 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-MED21 Antibody [PCRP-MED21-4B5] - BSA and Azide free (A278437).

hSrb7, MED21_HUMAN, Mediator complex subunit 21, Mediator of RNA polymerase II transcription subunit 21, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7, SRB7 suppressor of RNA polymerase B homolog, Surb7