Anti-MEK3 Antibody [ARC1933] (A305938)

Name: Anti-MEK3 Antibody [ARC1933]
Brand: Antibodies.com
SKU: A305938

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This product is discontinued

Anti-MEK3 Antibody [ARC1933] (A305938) has been discontinued and is no longer available.

View all Anti-MEK3 Antibodies.

Specifications

Name

Anti-MEK3 Antibody [ARC1933]

Description

Rabbit monoclonal [ARC1933] antibody to MEK3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-347 of human MEK3 (P46734).

Sequence

FYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1933

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AW212142, dual specificity mitogen activated protein kinase kinase 3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, map2k3, MAPK ERK kinase 3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, Mitogen activated protein kinase kinase 3, MKK 3, MKK3, mMKK3b, MP2K3_HUMAN, PRKMK 3, PRKMK3, protein kinase, mitogen-activated, kinase 3, SAPK kinase 2, SAPKK 2, SAPKK2, Stress activated protein kinase kinase 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-MEK3 Antibody [ARC1933] (A305938)

Western blot analysis of extracts of various cell lines, using Anti-MEK3 Antibody [ARC1933] (A305938) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-MEK3 Antibody [ARC1933] (A305938)

Immunofluorescence analysis of C6 cells using Anti-MEK3 Antibody [ARC1933] (A305938) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-MEK3 Antibody [ARC1933] (A305938)

Immunofluorescence analysis of NIH-3T3 cells using Anti-MEK3 Antibody [ARC1933] (A305938) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-MEK3 Antibody [ARC1933] (A305938)

Immunofluorescence analysis of U-2 OS cells using Anti-MEK3 Antibody [ARC1933] (A305938) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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