Anti-MEK5 Antibody (A15448)

Name: Anti-MEK5 Antibody
Brand: Antibodies.com
SKU: A15448
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to MEK5 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-MEK5 Antibody

Description

Rabbit polyclonal antibody to MEK5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MEK5 (NP_660143.1).

Sequence

MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dual specificity mitogen activated protein kinase kinase 5, Dual specificity mitogen-activated protein kinase kinase 5, EC 2.7.12.2, HsT17454, MAP kinase kinase 5, MAP kinase kinase MEK5b, MAP2K5, MAPK/ERK kinase 5, MAPKK 5, MAPKK5, MEK 5, mitogen-activated protein kinase kinase 5, MKK5, MP2K5_HUMAN, PRKMK5, Protein kinase, mitogen-activated, kinase 5, SAPKK5, SKK5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-MEK5 Antibody (A15448)

Western blot analysis of extracts of various cell lines, using Anti-MEK5 Antibody (A15448) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-MEK5 Antibody (A15448)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-MEK5 Antibody (A15448) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-MEK5 Antibody (A15448)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MEK5 Antibody (A15448) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-MEK5 Antibody (A15448)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-MEK5 Antibody (A15448) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.