MELK Cell Based ELISA Kit (A103738)

MELK Cell Based ELISA Kit

Cell-based (qualitative)

This MELK Cell Based ELISA Kit allows for the detection of MELK and the effects that certain stimulation conditions have on MELK expression in different cell lines. Qualitative determination of MELK concentration is achieved by an indirect ELISA format. In essence, the MELK is captured by Anti-MELK Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MELK Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human

Store at + 4°C. Stable for 6 months.

AI327312, hMELK, HPK 38, hPK38, KIAA0175, Likely ortholog of maternal embryonic leucine zipper kinase, Maternal embryonic leucine zipper kinase, MELK_HUMAN, mKIAA0175, MPK38, OTTHUMP00000021377, OTTHUMP00000046113, pEg3 kinase, Protein kinase Eg3, Protein kinase PK38, RP23 382O11.1, Tyrosine protein kinase MELK