Anti-METTL3 Antibody (A12244)

Name: Anti-METTL3 Antibody
Brand: Antibodies.com
SKU: A12244
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to METTL3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-METTL3 Antibody

Description

Rabbit polyclonal antibody to METTL3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).

Sequence

MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase, IME4, M6A, Methyltransferase like protein 3, Methyltransferase-like protein 3, mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase, mRNA m(6)A methyltransferase, MT-A70, MTA70, MTA70_HUMAN, N6 adenosine methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase 70 kDa subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-METTL3 Antibody (A12244)

Western blot analysis of various lysates, using Anti-METTL3 Antibody (A12244) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-METTL3 Antibody (A12244)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-METTL3 Antibody (A12244) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-METTL3 Antibody (A12244)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-METTL3 Antibody (A12244) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-METTL3 Antibody (A12244)

Confocal immunofluorescence analysis of Hela cells using Anti-METTL3 Antibody (A12244) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).