Anti-MGMT Antibody [MGMT/7454] - BSA and Azide free (A316692)

$765

Mouse monoclonal [MGMT/7454] antibody to MGMT for WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-MGMT Antibody [MGMT/7454] - BSA and Azide free
Description
Mouse monoclonal [MGMT/7454] antibody to MGMT.
Specificity
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6  alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
Applications
WB, IHC-P
Positive Control
Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).
Dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Purified His-tagged MGMT protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
MGMT/7454
Isotype
IgG2
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
21.646 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
6 O methylguanine DNA methyltransferase, 6-O-methylguanine-DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT_HUMAN, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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