Anti-mH2A1 Antibody [ARC1396] (A306292)

Name: Anti-mH2A1 Antibody [ARC1396]
Brand: Antibodies.com
SKU: A306292
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC1396] antibody to mH2A1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-mH2A1 Antibody [ARC1396]

Description

Rabbit monoclonal [ARC1396] antibody to mH2A1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human macroH2A.1 (O75367).

Sequence

IHSEISNLAGFEVEAIINPTNADIDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLAL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1396

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Core histone macro h2a.1, Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2afy, H2AY_HUMAN, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, macroH2A.1, Macroh2a1, MACROH2A1.1, MacroH2A1.2, Medulloblastoma antigen MU MB 50.205, Medulloblastoma antigen MU-MB-50.205, mH2a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-mH2A1 Antibody [ARC1396] (A306292)

Western blot analysis of extracts of various cell lines, using Anti-mH2A1 Antibody [ARC1396] (A306292) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-mH2A1 Antibody [ARC1396] (A306292)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-mH2A1 Antibody [ARC1396] (A306292) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-mH2A1 Antibody [ARC1396] (A306292)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-mH2A1 Antibody [ARC1396] (A306292) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-mH2A1 Antibody [ARC1396] (A306292)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-mH2A1 Antibody [ARC1396] (A306292) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.