Anti-Midkine Antibody (A12574)

Name: Anti-Midkine Antibody
Brand: Antibodies.com
SKU: A12574
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Midkine for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Midkine Antibody

Description

Rabbit polyclonal antibody to Midkine.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-129 of human MDK (NP_002382.1).

Sequence

SECAEWAWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFENWGACDGGTGTKVRQGTLKKARYNAQCQETIRVTKPCTPK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Amphiregulin associated protein, Amphiregulin-associated protein, ARAP, FLJ27379, Mdk, Midgestation and kidney protein, MK, MK 1, MK1, MK_HUMAN, NEGF 2, NEGF2, Neurite growth promoting factor 2, Neurite outgrowth promoting protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, Retinoic acid inducible factor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Midkine Antibody (A12574)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-Midkine Antibody (A12574) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Midkine Antibody (A12574)

Immunofluorescence analysis of HeLa cells using Anti-Midkine Antibody (A12574) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Midkine Antibody (A12574)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Midkine Antibody (A12574) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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