Anti-MiTF Antibody [PCRP-MITF-1D9] - BSA and Azide free (A278298)

Mouse monoclonal [PCRP-MITF-1D9] antibody to MiTF for IP, IF, Flow Cytometry and WB.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-MiTF Antibody [PCRP-MITF-1D9] - BSA and Azide free
Description
Mouse monoclonal [PCRP-MITF-1D9] antibody to MiTF.
Specificity
MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.
Applications
IP, IF, Flow Cytometry, WB
Dilutions
IP: 1-2µg / 100-500µg proteins, IF: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, WB: 1-2 µg/ml
Reactivity
Human
Cross Reactivity
This antibody is predicted to cross react with Chicken, Mouse, Rat, Xenopus, and Zebrafish.
Immunogen
Recombinant full-length human MiTF protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-MITF-1D9
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
52-56 kDa (doublet)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-MiTF Antibody [PCRP-MITF-1D9] (A277710).
Synonyms
bHLHe32, Class E basic helix-loop-helix protein 32, CMM8, Homolog of mouse microphthalmia, Mi, Microphthalmia associated transcription factor, Microphthalmia, mouse, homolog of, Microphthalmia-associated transcription factor, mitfa, MITF_HUMAN, nacre, WS2, WS2A, z3A.1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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