Principle of Assay
This kit provides two stains - an Orange fluorescent dye (Ex/Em = 579/599 nm) and blue-fluorescent Hoechst 33342 (Ex/Em: 350/461 nm, when bound to DNA) for highly selective mitochondrial and nuclear staining, respectively, in live, GFP–transfected cells. Both dyes are retained after formaldehyde fixation and permeabilization. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
