Principle of Assay
This kit is designed to label mitochondria of live cells. This kit uses a proprietary mitochondrial green fluorescent probe (Ex/Em=490/523 nm), which, unlike other probes, appears to localize to mitochondria much less dependent on mitochondrial membrane potential. The dye, a hydrophobic compound, has the added advantage that it is essentially nonfluorescent in aqueous solutions and only becomes fluorescent once it accumulates in the lipid environment of mitochondria, and can stain live cells and formaldehyde-fixed cells, but after permeabilized, the fluorescent signal will be lost. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
