Anti-MLH1 Antibody [AMC0417] (A309932)

Name: Anti-MLH1 Antibody [AMC0417]
Brand: Antibodies.com
SKU: A309932

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This product is discontinued

Anti-MLH1 Antibody [AMC0417] (A309932) has been discontinued and is no longer available.

View all Anti-MLH1 Antibodies.

Specifications

Name

Anti-MLH1 Antibody [AMC0417]

Description

Mouse monoclonal [AMC0417] antibody to MLH1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human MLH1.

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0417

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309932)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MLH1 Antibody [AMC0417] (A309932) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 2: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309932)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control samples) using Anti-MLH1 Antibody [AMC0417] (A309932) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309932)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MLH1 Antibody [AMC0417] (A309932) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [AMC0417] (A309932)

Immunohistochemistry analysis of paraffin-embedded human pancreas using Anti-MLH1 Antibody [AMC0417] (A309932) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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