Figure 1: Western Blot - Anti-MMP3 Antibody [ARC51098] (A309113)
Western blot analysis of extracts of U-87MG cells, using Anti-MMP3 Antibody [ARC51098] (A309113) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Figure 2: Western Blot - Anti-MMP3 Antibody [ARC51098] (A309113)
Western blot analysis of extracts of Mouse lung, using Anti-MMP3 Antibody [ARC51098] (A309113) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MMP3 Antibody [ARC51098] (A309113) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-MMP3 Antibody [ARC51098] (A309113) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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