Principle of Assay
Mouse ZNF179 / BFP ELISA Kit (A77750) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse ZNF179 / BFP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for ZNF179 / BFP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ZNF179 / BFP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-ZNF179 / BFP Antibody, which binds the captured ZNF179 / BFP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of ZNF179 / BFP captured in each well. The concentration of ZNF179 / BFP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.