Mouse CD30L ELISA Kit (A532)

Mouse CD30L ELISA Kit

Sandwich (quantitative)

This CD30L enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-CD30L Antibody. Standards or samples are then added to the microtire plate wells and CD30L, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of CD30L present in the sample, Anti-CD30L Antibody (Biotin) is added to each well to "sandwich" the CD30L immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD30L will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, body fluids, tissue lysates and cell culture supernatants.

< 6 pg/ml

15.6-1000 pg/ml

Mouse

Store at 2-8°C for 4 months or at -20°C for 8 months.

CD153, CD153 antigen, CD30 antigen ligand, CD30 L, CD30 ligand, CD30-L, CD30LG, MGC138144, TNFL8_HUMAN, Tnfsf8, Tumor necrosis factor (ligand) superfamily member 8, Tumor necrosis factor ligand superfamily member 8