Principle of Assay
Mouse CDKN2A / p19ARF ELISA Kit (A74708) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse CDKN2A / p19ARF in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CDKN2A / p19ARF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CDKN2A / p19ARF present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CDKN2A / p19ARF Antibody, which binds the captured CDKN2A / p19ARF present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CDKN2A / p19ARF captured in each well. The concentration of CDKN2A / p19ARF can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.