Mouse Creatinine ELISA Kit (A73987)

This product is discontinued

Mouse Creatinine ELISA Kit (A73987) has been discontinued and is no longer available.

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Name
Mouse Creatinine ELISA Kit
Assay Type
Competitive
Principle of Assay
This Mouse Creatinine ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of Creatinine in plasma, tissue homogenates and other biological fluids.

A microplate has been precoated with Creatinine. Samples, standards and Anti-Creatinine Antibody (Biotin) are added to the wells, where Creatinine in the sample competes with the fixed Creatinine to bind to the antibody. The wells are washed to remove unbound material and HRP-conjugated streptavidin is added. After incubation, the wells are washed again and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of Creatinine in the sample and the intensity is measured at 450 nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Plasma, tissue homogenates and other biological fluids.
Sensitivity
< 0.75 nmol/ml
Product Range
1.25 - 80 nmol/ml
Reactivity
Mouse
Storage
4°C for 6 months.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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