The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.058 ng/ml

Product Range

0.156 - 10 ng/ml

Assay Time

1 - 4.5 Hours

Reactivity

Mouse

Synonyms

AACRYA, Alpha B Crystallin, Alpha crystallin B chain, Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, Cryab, CRYAB_HUMAN, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.