Mouse GHR ELISA Kit (A323203)

Mouse GHR ELISA Kit

Mouse GHR ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GHR in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Mouse GHR ELISA Kit (A323203) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse GHR in serum, plasma, and other biological fluids. An antibody specific for GHR has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GHR present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GHR Antibody, which binds the captured GHR present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GHR captured in each well. The concentration of GHR can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

18.75 pg/ml

31.25-2000 pg/ml

Mouse

No significant cross-reactivity or interference between mouse GHR and analogues was observed.

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

GH receptor, GH-binding protein, GHBP, GHBP, included, GHR_HUMAN, Growth hormone binding protein, Growth hormone receptor, Growth hormone receptor precursor, Growth hormone-binding protein, Growth hormone-binding protein, included, Increased responsiveness to growth hormone, included, Serum binding protein, Serum-binding protein, Somatotropin receptor