Mouse HGF ELISA Kit (A323202)

Mouse HGF ELISA Kit

Mouse HGF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse HGF in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Mouse HGF ELISA Kit (A323202) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse HGF in serum, plasma, and other biological fluids. An antibody specific for HGF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HGF present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HGF Antibody, which binds the captured HGF present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HGF captured in each well. The concentration of HGF can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.94 ng/ml

1.56-100 ng/ml

Mouse

No significant cross-reactivity or interference between mouse HGF and analogues was observed.

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A; scatter factor), Hepatocyte growth factor beta chain, Hepatocyte growth factor precursor, Hepatopoietin A, Hepatopoietin-A, HGFB, HGF_HUMAN, HPTA, Lung fibroblast derived mitogen , OTTHUMP00000161349, OTTHUMP00000206710, OTTHUMP00000206711, OTTHUMP00000206712, OTTHUMP00000206713, OTTHUMP00000206730, Scatter factor, SF