This antibody does not cross-react with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. This antibody may slightly cross react with mouse IgG2b and goat IgG.
Immunogen
Mouse IgG.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM106
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Ig gamma, Ig gamma 1 chain C region, Ig gamma 2 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-1 chain C region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Figure 1: Western Blot - Anti-Mouse IgG Antibody [RM106] (A121356)
Western blot of non-reduced (-) and reduced (+) mouse IgG1 (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG Antibody [RM106]. This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced ?1 form (~50 kDa).
ELISA of mouse immunoglobulins shows Anti-Mouse IgG Antibody [RM106] reacts to the Fc region of mouse IgG1. There is no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1. The plate was coated with different amounts of mouse IgG1. A serial dilution of Anti-Mouse IgG Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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