ELISA of mouse immunoglobulins shows Anti-Mouse IgG Antibody [RMG07] reacts to mouse IgG1, IgG2a, IgG2b, and IgG3. There is no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50ng/well of different immunoglobulins. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Mouse IgG Antibody [RMG07] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Anti-Mouse IgG Antibody [RMG07] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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