Mouse IgG2a kappa Isotype Control [MOPC-173] (FITC) (A86311)

Mouse IgG2a kappa Isotype Control [MOPC-173] (FITC) for Flow Cytometry.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Mouse IgG2a kappa Isotype Control [MOPC-173] (FITC)
Specificity
This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
Applications
Flow Cytometry
Dilutions
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Cross Reactivity
This antibody does not cross react with Mouse, Rat or Human.
Immunogen
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Host
Mouse
Clonality
Monoclonal
Clone ID
MOPC-173
Isotype
IgG2a
Light Chains
kappa
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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