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Recombinant Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)

Western Blot - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.com ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.com ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121332) - Antibodies.com
$480

Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b (Biotin) for IP, ICC, IHC, Flow Cytometry, ELISA and WB.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-Mouse IgG2b Antibody [RM108] (Biotin)
Description
Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b (Biotin).
Host Species
Rabbit
Target Species
Mouse
Specificity
This antibody recognizes the Fc region of mouse IgG2b.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA, WB
Recommended Dilutions
ELISA: 0.005-0.2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml
Immunogen
Mouse IgG2b.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein A chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
Western blot of non-reduced (-) and reduced (+) mouse IgG2b (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG2b Antibody [RM108] (Biotin). This antibody reacts to non-reduced IgG2b (~150 kDa) much stronger than the reduced ?2b form (~50 kDa).
ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.comEnlarge Image
Figure 2: Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
ELISA of mouse immunoglobulins shows Anti-Mouse IgG2b Antibody [RM108] reacts to the Fc region of mouse IgG2b. There is no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121332) - Antibodies.comEnlarge Image
Figure 3: Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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