A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of Anti-Mouse IgM Antibody [RM109] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of mouse immunoglobulins shows Anti-Mouse IgM Antibody [RM109] reacts to mouse IgM. There is no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgM Antibody [RM109] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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