Mouse Leptin ELISA Kit (A1596)

Mouse Leptin ELISA Kit

Sandwich (quantitative)

Mouse Leptin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Leptin in the samples. A monoclonal antibody specific for mouse Leptin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Leptin, and Leptin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Leptin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

This kit can assay both natural and recombinant mouse Leptin.

Mouse

This assay has shown no significant cross-reactivity.

Store at 2 - 8°C.

FLJ94114, LEP, LEPD, Leptin (murine obesity homolog), Leptin (obesity homolog, mouse), Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, LEP_HUMAN, OB, Obese protein, Obese, mouse, homolog of, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS, OTTHUMP00000212285

This product is for research use only. It is not intended for diagnostic or therapeutic use.