Mouse Osteopontin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse OPN in the samples. A monoclonal antibody specific for mouse OPN has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for OPN, and OPN present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of OPN bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

0.05 pg/ml

Product Range

15.63 - 1000 pg/ml

Specificity

This kit can assay both natural and recombinant mouse OPN.

Reactivity

Mouse

Cross Reactivity

This assay has shown no significant cross-reactivity.

Storage

Store at 2 - 8°C.

Synonyms

BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP_HUMAN, PSEC0156, Secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.