Mouse Pleiotrophin ELISA Kit (A6548)

Mouse Pleiotrophin ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

24 pg/ml

78.125 - 5000 pg/ml

1 - 4.5 Hours

Mouse

HARP, HB-GAM, HBBM, HBGAM, HBGF-8, HBGF8, HBNF, HBNF-1, HBNF1, heparin affin regulatory protein, Heparin binding growth associated molecule, Heparin binding growth factor 8, Heparin binding neurite outgrowth promoting factor 1, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, heparin-binding neurite outgrowth promoting factor, Heparin-binding neurite outgrowth-promoting factor 1, NEGF1, Neurite growth promoting factor 1, Neurite outgrowth-promoting factor, heparin-binding, OSF-1, OSF1, Osteoblast-specific factor 1, pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), PTN, PTN_HUMAN