Mouse VEGF Receptor 2 ELISA Kit (A323237)

Mouse VEGF Receptor 2 ELISA Kit

Mouse VEGF Receptor 2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse VEGF Receptor 2 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Mouse VEGF Receptor 2 ELISA Kit (A323237) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse VEGF Receptor 2 in serum, plasma, and other biological fluids. An antibody specific for VEGF Receptor 2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the VEGF Receptor 2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-VEGF Receptor 2 Antibody, which binds the captured VEGF Receptor 2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of VEGF Receptor 2 captured in each well. The concentration of VEGF Receptor 2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.08 ng/ml

0.16-10 ng/ml

Mouse

No significant cross-reactivity or interference between mouse VEGF Receptor 2 and analogues was observed.

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

CD309, CD309 antigen, EC 2.7.10.1, Fetal liver kinase 1, FLK-1, FLK1, FLK1, mouse, homolog of, Kdr, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR-2, VEGFR2, VGFR2_HUMAN