Anti-MRP4 Antibody [ARC52221] (A308776)

Name: Anti-MRP4 Antibody [ARC52221]
Brand: Antibodies.com
SKU: A308776

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This product is discontinued

Anti-MRP4 Antibody [ARC52221] (A308776) has been discontinued and is no longer available.

View all Anti-MRP4 Antibodies.

Specifications

Name

Anti-MRP4 Antibody [ARC52221]

Description

Rabbit monoclonal [ARC52221] antibody to MRP4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1067-1325 of human MRP4/ABCC4 (NP_005836.2).

Sequence

SQEKVGIVGRTGAGKSSLISALFRLSEPEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALQEVQLKETIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILRKNQILIIDEATANVDPRTDELIQKKIREKFAHCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYVLLQNKESLFYKMVQQLGKAEAAALTETAKQVYFKRNYPHIGHTDHMVTNTSNGQPSTLTIFETAL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52221

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

140 - 200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ABCC 4, ABCC4, ATP binding cassette sub family C (CFTR/MRP) member 4, ATP binding cassette sub family C member 4, ATP-binding cassette sub-family C member 4, bA464I2.1, bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4), Canalicular multispecific organic anion transporter, Canalicular multispecific organic anion transporter ABC superfamily, EST170205, MOAT B, MOAT-B, MOATB, MRP 4, MRP/cMOAT related ABC transporter, MRP/cMOAT-related ABC transporter, MRP4_HUMAN, Multi specific organic anion transporter B, Multi-specific organic anion transporter B, Multidrug resistance associated protein 4, Multidrug resistance-associated protein 4, OTTHUMP00000018560

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-MRP4 Antibody [ARC52221] (A308776)

Western blot analysis of extracts from normal (control) and MRP4/ABCC4 knockout (KO) 293T(KO) cells, using Anti-MRP4 Antibody [ARC52221] (A308776) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-MRP4 Antibody [ARC52221] (A308776)

Western blot analysis of Rat kidney, using Anti-MRP4 Antibody [ARC52221] (A308776) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Western Blot - Anti-MRP4 Antibody [ARC52221] (A308776)

Western blot analysis of Mouse kidney, using Anti-MRP4 Antibody [ARC52221] (A308776) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 4: Immunohistochemistry - Anti-MRP4 Antibody [ARC52221] (A308776)

Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Anti-MRP4 Antibody [ARC52221] (A308776) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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