Anti-MUC16 Antibody [X325] (A282007)

Anti-MUC16 Antibody [X325]

Mouse monoclonal [X325] antibody to MUC16.

This antibody recognises the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. recognises the epitope specificity group B, similar to the M-11 antibody. Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.

ELISA, WB, IHC-P

Human

CA125 antigen purified from human ovarian carcinoma.

Mouse

Monoclonal

X325

IgG1

Unconjugated

Ion exchange chromatography of ascites.

Liquid

Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CA125 antibody, clone X325 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X325 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.

CA 125, CA-125, CA125, CA125 ovarian cancer antigen, Cancer antigen 125, FLJ14303, MUC 16, MUC-16, MUC16_HUMAN, Mucin 16, Mucin 16 cell surface associated, Mucin-16, Mucin16, Ovarian cancer related tumor marker CA125, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125