Anti-Myelin Basic Protein Antibody [7D2] (A85329)

Immunofluorescent analysis rat brain hippocampal section stained with Anti-MBP Antibody (1:5,000 | green) and Anti-NF-M Antibody (A85323 | 1:2,000 | red). The Anti-MBP Antibody stains myelin sheathes around axons, while the Anti-NF-M Antibody labels dendrites and axons of neuronal cells.

Rat mixed neuron/glial cultures stained with Anti-MBP Antibody (red) and Anti-NF-L Antibody (A85286 | green). Blue is a DNA stain. Note that the MBP antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for MBP, though the neuronal processes can be seen with the NF-L antibody.

Western blot analysis of different tissue lysates using Anti-MBP Antibody (1:10,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord. Bands at 21.5kDa and 18.5kDa are the two larger transcripts from the MBP gene, showing that the epitope of this antibody depends on the sequence encoded by exon 2. Note that the Anti-MBP Antibody binds all four rat MBP transcripts running at 21.5kDa, 18.5kDa, 17kDa and 14kDa, so that this antibody binds to the core sequence of human and rodent MBP.

Blots of 20µg crude rat brain homogenate blotted with two different MBP antibodies. Lane 1: Anti-MBP Antibody (A85322 | 1:5,000). Lane 2: Anti-MBP Antibody (A85329 | 1:5,000). Anti-MBP Antibody (A85322) binds all four transcripts: 21.5kDa,18.5kDa, 17kDa and 14kDa. While Anti-MBP Antibody (A85329) binds the largest 21.5 kDa and 18.5 kDa transcripts preferentially.