General Notes
Mouse anti Human myeloperoxidase antibody, clone D08-8G5, recognizes myeloperoxidase (MPO), a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is largely expressed by neutrophils within azurophilic granules, but is also present in monocytes, macrophages and central nervous microglial cells (Strzepa et al. 2017). MPO is synthesized as a precursor protein that undergoes a series of modifications including cleavage and glycosylation to form the catalytically inactive MPO precursor. Subsequently an iron-heme is incorporated into the protein resulting in the catalytically active MPO (Hansson et al. 2006). In response to bacterial infection, neutrophils containing MPO fuse with the phagosome and when the common membrane is ruptured, MPO is release into the phagosome. MPO then uses H2O2 to generate the bactericidal compound, hypochlorous acid (HClO) (Strzepa et al. 2017). MPO deficiency is associated with a higher occurrence of severe infections and chronic inflammatory processes, although ~50% of individuals are asymptomatic (Antachopoulos 2010).The biotinylated Mouse anti Human myeloperoxidase antibody, clone D08-8G5 (MCA6261B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human myeloperoxidase antibody, clone D02-2A1 (MCA6260GA) as the capture antibody.
