Anti-Myh14 Antibody [ARC53864] (A305326)

Name: Anti-Myh14 Antibody [ARC53864]
Brand: Antibodies.com
SKU: A305326

Enlarge Image

This product is discontinued

Anti-Myh14 Antibody [ARC53864] (A305326) has been discontinued and is no longer available.

View all Anti-Myh14 Antibodies.

Specifications

Name

Anti-Myh14 Antibody [ARC53864]

Description

Rabbit monoclonal [ARC53864] antibody to Myh14.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MYH14 (NP_079005.3).

Sequence

AGKVDYKALMKNMDPLNDNVAALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53864

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2400004E04Rik, DFNA4, DKFZp667A1311, FLJ13881, FLJ43092, FP17425, II C, KIAA2034, MHC16, Myh 14, MYH14_HUMAN, Myosin, Myosin 14, Myosin heavy chain, Myosin heavy chain 14, Myosin heavy chain non muscle IIc, Myosin heavy polypeptide 14, Myosin-14, NMHC II C, NMHC II-C, Non muscle myosin heavy chain IIc, non-muscle IIc, Non-muscle myosin heavy chain IIc, Nonmuscle myosin heavy chain II C, OTTMUSP00000019210

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-Myh14 Antibody [ARC53864] (A305326)

Western blot analysis of extracts of Mouse lung, using Anti-Myh14 Antibody [ARC53864] (A305326) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Western Blot - Anti-Myh14 Antibody [ARC53864] (A305326)

Western blot analysis of extracts of various cell lines, using Anti-Myh14 Antibody [ARC53864] (A305326) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-Myh14 Antibody [ARC53864] (A305326)

Immunofluorescence analysis of HT-29 using Anti-Myh14 Antibody [ARC53864] (A305326) at a dilution of 1:100(40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-Myh14 Antibody [ARC53864] (A305326)? Please let us know so that we can list the citation on this page.