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Anti-MYH2 Antibody (A88759)

Western Blot - Anti-MYH2 Antibody (A88759) - Antibodies.com Western Blot - Anti-MYH2 Antibody (A88759) - Antibodies.com Immunofluorescence - Anti-MYH2 Antibody (A88759) - Antibodies.com Immunofluorescence - Anti-MYH2 Antibody (A88759) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to MYH2 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-MYH2 Antibody
Description
Rabbit polyclonal antibody to MYH2.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1).
Sequence
SFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
adult 2, Fast 2a myosin heavy chain, IBM3, Inclusion body myopathy 3, autosomal dominant, MYH2A, MYH2_HUMAN, MYHas8, MyHC IIa, MyHC-2a, MyHC-IIa, MYHSA2, Myosin heavy chain, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, Myosin heavy chain skeletal muscle adult 2, Myosin heavy polypeptide 2 skeletal muscle adult, Myosin-2, MYPOP, skeletal muscle, Type IIA myosin heavy chain
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-MYH2 Antibody (A88759) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-MYH2 Antibody (A88759)
Western blot analysis of extracts of Rat skeletal muscle, using Anti-MYH2 Antibody (A88759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-MYH2 Antibody (A88759) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-MYH2 Antibody (A88759)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-MYH2 Antibody (A88759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-MYH2 Antibody (A88759) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-MYH2 Antibody (A88759)
Immunofluorescence analysis of mouse skeletal muscle cells using Anti-MYH2 Antibody (A88759) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-MYH2 Antibody (A88759) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-MYH2 Antibody (A88759)
Immunofluorescence analysis of rat skeletal muscle cells using Anti-MYH2 Antibody (A88759) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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