This antibody recognises human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.

Applications

WB, IHC-P, ELISA

Dilutions

ELISA: 1:5,000 - 1:20,000, IHC-P: 2.5 µg/ml, WB: 1:500 - 1:2,000

Reactivity

Human, Birds, Mammals, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.

Sequence

KKRPQRATSNVFAMFD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

General Notes

Rabbit anti Human myosin regulatory light chain antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.

Synonyms

20 kDa myosin light chain, Human 20kDa myosin light chain (MLC2) mRNA complete cds, LC20, MGC3505, MLC 2, MLC-2C, MLC2, MLY 9, MRLC1, MYL9_HUMAN, Myosin light chain 9 regulatory, Myosin light polypeptide 9 regulatory, myosin regulatory light chain 1, Myosin regulatory light chain 2, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, Myosin RLC, Myosin vascular smooth muscle light chain 2, MYRL2, OTTHUMP00000030857, smooth muscle isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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