Anti-Myosin Antibody (A88758)

Name: Anti-Myosin Antibody
Brand: Antibodies.com
SKU: A88758
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Myosin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Myosin Antibody

Description

Rabbit polyclonal antibody to Myosin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1450-1550 of human MYH4 (NP_060003.2).

Sequence

QRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

adult 1, MYH1, MYH1_HUMAN, MYH2, MYH3, MYH4, MYH6, MyHC-2x, MyHC-IIx/d, MYL1, MYL2, MYL3, MYL5, MYL6, Myosin 1, Myosin heavy chain, Myosin heavy chain 1, Myosin heavy chain 1 skeletal muscle adult, Myosin heavy chain 2 skeletal muscle adult, Myosin heavy chain 2x, Myosin heavy chain 3 skeletal muscle adult, Myosin heavy chain 4 skeletal muscle adult, Myosin heavy chain 6 skeletal muscle adult, Myosin heavy chain IIx/d, Myosin heavy chain skeletal muscle adult 1, Myosin II, Myosin light chain 1 alkali skeletal fast, Myosin light chain 2 regulatory cardiac slow, Myosin light chain 3 alkali ventricular skeletal slow, Myosin light chain 4 alkali atrial embryonic, Myosin light chain 5 regulatory, Myosin light chain 6 alkali smooth muscle and non muscle, Myosin-1, Myosin1, skeletal muscle

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Myosin Antibody (A88758)

Western blot analysis of Mouse skeletal muscle, using Anti-Myosin Antibody (A88758) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Myosin Antibody (A88758)

Western blot analysis of various lysates, using Anti-Myosin Antibody (A88758) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-Myosin Antibody (A88758)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Myosin Antibody (A88758) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Myosin Antibody (A88758)

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using Anti-Myosin Antibody (A88758) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.