Anti-Myosin Light Chain 2 Antibody (A14786)

Name: Anti-Myosin Light Chain 2 Antibody
Brand: Antibodies.com
SKU: A14786

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This product is discontinued

Anti-Myosin Light Chain 2 Antibody (A14786) has been discontinued and is no longer available.

View all Anti-Myosin Light Chain 2 Antibodies.

Specifications

Name

Anti-Myosin Light Chain 2 Antibody

Description

Rabbit polyclonal antibody to Myosin Light Chain 2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).

Sequence

MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cardiac myosin light chain-2, Cardiac ventricular myosin light chain 2, CMH10, MLC 2v, MLC-2, MLC-2v, MLC2, MLRV_HUMAN, MYL 2, MYL2, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin, RLC of myosin, Slow cardiac myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Myosin Light Chain 2 Antibody (A14786)

Western blot analysis of extracts of various cell lines, using Anti-Myosin Light Chain 2 Antibody (A14786) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-Myosin Light Chain 2 Antibody (A14786)

Immunofluorescence analysis of rat heart using Anti-Myosin Light Chain 2 Antibody (A14786) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Myosin Light Chain 2 Antibody (A14786)

Immunofluorescence analysis of mouse heart using Anti-Myosin Light Chain 2 Antibody (A14786) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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