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Anti-NCAPD2 Antibody (A305867)

Western Blot - Anti-NCAPD2 Antibody (A305867) - Antibodies.com Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.com Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.com Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.com
This product is discontinued

Anti-NCAPD2 Antibody (A305867) has been discontinued and is no longer available.

View all Anti-NCAPD2 Antibodies.

Name
Anti-NCAPD2 Antibody
Description
Rabbit polyclonal antibody to NCAPD2.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1250-1350 of human NCAPD2 (NP_055680.3).
Sequence
FGDKLSDESIFSAFLSVVGKLRRGAKPEGKAIIDEFEQKLRACHTRGLDGIKELEIGQAGSQRAPSAKKPSTGSRYQPLASTASDNDFVTPEPRRTTRRHP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
175 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2810406C15Rik, 2810465G24Rik, CAP-D2, CAPD2, CEP2, CEP250, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, CNAP1, CND1, Condensin, Condensin complex subunit 1, eg7, hCAP-D2, hCAPD2, KIAA0159, mKIAA0159, non-SMC condensin I complex, subunit D2, XCAP D2 homolog
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-NCAPD2 Antibody (A305867) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-NCAPD2 Antibody (A305867)
Western blot analysis of extracts of various cell lines, using Anti-NCAPD2 Antibody (A305867) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-NCAPD2 Antibody (A305867)
Immunofluorescence analysis of C6 cells using Anti-NCAPD2 Antibody (A305867) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-NCAPD2 Antibody (A305867)
Immunofluorescence analysis of L929 cells using Anti-NCAPD2 Antibody (A305867) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-NCAPD2 Antibody (A305867) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-NCAPD2 Antibody (A305867)
Immunofluorescence analysis of U2OS cells using Anti-NCAPD2 Antibody (A305867) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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