Anti-NCX1 Antibody (A9718)

Name: Anti-NCX1 Antibody
Brand: Antibodies.com
SKU: A9718
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NCX1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NCX1 Antibody

Description

Rabbit polyclonal antibody to NCX1.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:6,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 600-700 of human SLC8A1 (NP_066920.1).

Sequence

DEIVKTISVKVIDDEEYEKNKTFFLEIGEPRLVEMSEKKALLLNELGGFTITGKYLFGQPVFRKVHAREHPILSTVITIADEYDDKQPLTSKEEEERRIAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CNC, DKFZp779F0871, MGC119581, Na(+)/Ca(2+)-exchange protein 1, Na+/Ca2+ exchange protein 1, Na+/Ca2+ exchanger, NAC1_HUMAN, NCX, NCX 1, SLC8A1, SLC8A1 protein, Sodium Calcium Exchanger, Sodium/calcium exchanger 1, Solute carrier family 8 (sodium/calcium exchanger) member 1, Solute carrier family 8 member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NCX1 Antibody (A9718)

Western blot analysis of extracts of various cell lines, using Anti-NCX1 Antibody (A9718) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-NCX1 Antibody (A9718)

Western blot analysis of extracts of Mouse brain, using Anti-NCX1 Antibody (A9718) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-NCX1 Antibody (A9718)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-NCX1 Antibody (A9718) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NCX1 Antibody (A9718)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-NCX1 Antibody (A9718) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.