Anti-Ndufs1 Antibody [ARC52613] (A309039)

Name: Anti-Ndufs1 Antibody [ARC52613]
Brand: Antibodies.com
SKU: A309039

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This product is discontinued

Anti-Ndufs1 Antibody [ARC52613] (A309039) has been discontinued and is no longer available.

View all Anti-Ndufs1 Antibodies.

Specifications

Name

Anti-Ndufs1 Antibody [ARC52613]

Description

Rabbit monoclonal [ARC52613] antibody to Ndufs1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human NDUFS1 (NP_004997.4).

Sequence

VEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52613

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa / 79 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CI-75kD, Complex I 75Kd, Complex I, mitochondrial respiratory chain, 75 kD subunit, Complex I-75kD, mitochondrial, NADH coenzyme Q reductase, NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase), NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial, NADH-ubiquinone oxidoreductase 75 kDa subunit, NDUS1_HUMAN, PRO1304

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Ndufs1 Antibody [ARC52613] (A309039)

Western blot analysis of extracts of various cell lines, using Anti-Ndufs1 Antibody [ARC52613] (A309039) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Ndufs1 Antibody [ARC52613] (A309039)

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Figure 3: Immunohistochemistry - Anti-Ndufs1 Antibody [ARC52613] (A309039)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Ndufs1 Antibody [ARC52613] (A309039) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Ndufs1 Antibody [ARC52613] (A309039)

Immunohistochemistry analysis of paraffin-embedded human heart using Anti-Ndufs1 Antibody [ARC52613] (A309039) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.